WESTAR is Cyanagen’s patented product line dedicated to Western Blotting

  • WESTAR SUN – standard signal intensity
  • WESTAR NOVA 2.0 – medium signal intensity
  • WESTAR ETA C 2.0 – high signal intensity
  • WESTAR ETA C ULTRA 2.0 – very high signal intensity
  • WESTAR SUPERNOVA – extreme signal intensity

Westar products cover a wide range of possible signal intensity and duration, from the entry level ECL-SUN, to the top of the line, SUPERNOVA. Each product is at the top of its market segment for performance, and cost to performance ratio.

PRODUCT INTRODUCTION

WESTAR detection reagents are non-isotopic, luminol-based chemiluminescence substrate, designed for the chemiluminescent detection of immobilized proteins and immobilized nucleic acids conjugated with horseradish peroxidase (HRP). Using this method, it is possible to detect membrane immobilized specific antigens, or sequences of nucleic acids, labeled directly with HRP or indirectly with HRP-labeled antibodies/streptavidin.

 http://www.cyanagen.com/images/Prodotti/Western-blot-Elisa_file/Image003.png
Figure 1. WESTAR for Western blot.

WESTAR substrates for HRP detection of CYANAGEN are a powerful tool for studying low expressed protein, up to a detection limit of few femtograms. The higher and steadier light emission produced by these second generation of chemiluminescent substrates also give the possibility of using lower amounts of precious antibodies (primary and secondary), greatly reducing the cost for Western blot assay (see figure 2). 

Antobodies saving comparison 
Figure 2. Antibodies saving comparison.
Human Transferrin diluted (5 to 0.5ng) and electrophoresis performed. The gels were transferred to PVDF membranes, blocked and incubated with different dilutions of rabbit anti-transferrin. After washing, the membranes were incubated with different dilutions of HRP-conjugated goat anti-rabbit antibody. The membranes were washed again and then incubated with substrate. Blots were acquired with ImageQuant LAS 4000 (GEHC) for 300 sec.

COMPATIBLE IMAGING DEVICE

  • ImageQuant LAS-4000/Mini (GE Healthcare)
  • DIAS-II (SERVA)
  • ChemiDocXRS and VersaDoc (BIO-RAD)
  • ChemiImager (Alpha Innotech)
  • Image Station 2000/40000MM (Kodak)
  • FOTO/Analyst Luminary/FX Systems (Fotodyne)
  • LAS-3000 (Fujifilm)
  • UVIchemi and UVIprochemi (UVItec Ltd.)
  • G:BOX/GeneGnome (Syngene)
  • Odyssey FC (LI-COR)
  • C-DiGit Blot Scanner (LI-COR)

NOTE: This is just a brief summary of the main instrumentation compatible with WESTAR substrates.

 

ELISTAR for ELISA

Elisa substrates icon

ELISTAR is the Cyanagen’s NEW patented product line dedicated to ELISA.

  • ELISTAR SUN
  • ELISTAR NOVA 2.0
  • ELISTAR ETA C 2.0
  • ELISTAR ETA C ULTRA 2.0
  • ELISTAR SUPERNOVA

ELISTAR products offer the best performance in their market segment and are powerful tools in diagnostic research and food analysis.

 

Protein Quantitation

Cyanagen’s Protein Quantitation line includes Qpro and mQpro.
They are Bicinchoninic Acid (BCA) - based protein assay (BCA assay) for highly sensitive colorimetric analysis, compatible with detergent solubilized protein solutions.
The BCA method is fast and easy, providing enhanced flexibility with much greater tolerance to interferences by non-ionic detergents and buffer salts.

ADVANTAGES

  • Broad linear working ranges with excellent sensitivity
  • Can be used with tubes and microplates
  • Less protein-to-protein variation than dye-binding methods
  • Unaffected by most ionic and nonionic detergents such as Triton-100 and SDS (1%)
  • Much easier and faster than the classical Lowry method

 

PRODUCTS

Order-No

Product Description

Quantity

PRTD1,0500

QPRO – BCA kit Standard
20÷2000 mg/ml protein

  • Solution A: 500 mL of 1% BCA/tartrate in alkaline carbonate buffer
  • Solution B: 15 mL of 4% CuSO4 5H2O in water

PRTD2,0500

µQPRO – BCA kit Micro
0.5÷20 mg/ml protein

  • Solution A: 240 mL of tartrate in alkaline carbonate buffer
  • Solution B: 240 mL of 4% BCA in water
  • Solution C: 12 mL of 4% CuSO4 5H2O in water

 

RELATED PRODUCTS

Fluorescent in-gel protein stainFluorescent Gel staining icon

Ruby Gel Stain is a ready to use kit for rapid and sensitive protein staining in 1D and 2D SDS polyacrylamide electrophoresis gels.
It enables optimal visualization and quantitation of proteins with high contrast and the same sensitivity as silver staining without its drawbacks. The staining procedure is a simple 220 minutes, three step protocol.
After staining, proteins can be removed from the gel and analyzed by mass spectrometry without interference from the stain.
The dye has optimal excitation at 302 and 470 nm, with an emission maximum at approximately 610 nm. Ruby Gel Stain can be excited with UV-light, UV transilluminator and 405, 445, 473-488 nm laser sources.

FEATURES

  • Ready to use kit
  • High purity dye: >98%
  • Optimal signal to background ratio
  • Strong, uniform and reproducible signal from 0.2 ng to 10 ng protein
  • Fast staining protocol (220 min)
  • Convenient: fixing and destaining solutions included in the kit

The gel can be visualized using excitation wavelength in the 400-500 nm range with bright emission peak centred around 610 nm.

PRODUCTS

Order-No

Product description

Quantity

Sufficient for

PRTD066

Ruby Gel Stain

solution A 50 mL,
solution B 100 mL

solution A 50 ml, solution B 100 ml for 1 minigel (8,6 x 6,7 x 0,1 cm)

solution A 250 ml, solution B 500 ml

solution A 250 ml, solution B 500 ml for 5 minigel (8,6 x 6,7 x 0,1 cm)

Green nucleic acid stain  

 

DNA/RNA DETECTION

Green Stain

Features:

Fluorescent dyes iconGreen Stain is a versatile and easy to use tool for the detection of nucleic acids (dsDNA, ssDNA, RNA) in electrophoretic gels. Bright green fluorescent bands and very low background fluorescence are its major features, together with high sensitivity and very low toxicity.
The nucleic acid-bound Green Stain is efficiently excited at ~254 nm and ~488 nm; it can also be excited at ~312 nm with loss of sensitivity. Detection can be performed with the same instruments used for ethidium bromide and SYBR® Green gel staining such as standard UV transilluminator (254 nm) as well as with CCD-camera imaging system or laser-based scanner selecting the SYBR® Green filter.

Sufficient for:

0.5 ml is sufficient for 50 minigels stain

Components:

10000X Solution in DMSO

Storage:

-20°C

INTRODUCTION

Cyanagen has developed a new fluorescent dye for the detection of nucleic acids: Green Stain (10000X in DMSO).
Green Stain is a versatile and easy to use tool for the detection of nucleic acids (dsDNA, ssDNA, RNA) in electrophoretic gels, based on pre-electrophoresis gel staining, sample pre-staining and post-electrophoresis gel staining.
Excitation/emission spectra of Green Stain bound to dsDNA
Excitation/emission spectra of Green Stain bound to dsDNA

FEATURES

  • Sensitivity: Detection as little as 100 pg dsDNA per band.
  • High contrast: Bright green fluorescence with exceptionally low background.
  • Safe: Very low toxicity.
  • Easy to use: Substitute your reagent with Green Stain using one of the following protocols:
    - pre-electrophoresis gel staining for the highest sensitivity
    - sample pre-staining for the best migration results
    - post-electrophoresis gel staining for particular protocol needs

PRODUCTS

Order-No

Product Description

Quantity

NAGS068

Green Stain

0,5 ml
1,0 ml (2 x 0,5 ml)

Stripping buffer

INTRODUCTION

Renew Stripping Buffer is a ready-to-use solution allowing primary and secondary antibodies to be quickly removed from nitrocellulose and PVDF membranes and, as a result, Western blot to be reprobed with different antibodies.
Renew Stripping Buffer is suitable for all the chemiluminescent substrates and ideal if used together with WESTAR.

FEATURES

Effective: robust formulation for a fast stripping of most antibodies
Gentle: does not damage target proteins on the blots
Saves time: no need to re-run gels and blots
Saves sample: re-probe the membrane using the same target protein
Safe: non toxic and odorless formulation.

PRODUCTS

Order-No

Product Description

Quantity

SBS069

Renew Stripping Buffer

50 mL

250 mL

500 mL (2 x 250 mL)

Back to Products